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Intravital microscopy provides dynamic understanding of multiple cell biological processes, but its limited resolution has so far precluded structural analysis. Because it is difficult to capture rare and transient events, only a few attempts have been made to observe specific developmental and pathological processes in animal models using electron microscopy. The multimodal correlative approach that we propose here combines intravital microscopy, microscopic X-ray computed tomography and three-dimensional electron microscopy…
Based on landmarks, the IVM volume was registered into the microCT dataset with Amira Software, which enabled precise determination of the position of the tumor cell within the resin block and relative to its surface.
For Research Use Only. Not for use in diagnostic procedures.