Thermo Fisher Scientific › Electron Microscopy › Electron Microscopes › 3D Visualization, Analysis and EM Software › Use Case Gallery
Thermo Fisher Scientific › Electron Microscopy › Electron Microscopes › 3D Visualization, Analysis and EM Software › Use Case Gallery
The liver contains a variety of vessels and participates in miscellaneous physiological functions. While past studies generally focused on certain hepatic vessels, we simultaneously obtained all the vessels and cytoarchitectural information of the intact mouse liver lobe at single-cell resolution. […] providing a technology roadmap for studying the fine hepatic vascular structures and their spatial relationship, which will help research into liver diseases and evaluation of medical efficacies in the future.
In this article, we used Tek-Cre*Ai47 mice to acquire the vessel structures and cytoarchitecture information of intact liver lobe with single-cell resolution, based on propidium iodide staining and the HD-fMOST system. According to the morphology discrepancies among different vessels, we segmented large-caliber vessels, namely portal vein, hepatic vein, hepatic artery, intrahepatic bile ducts, and lymphatic vessels, and reconstructed hepatic sinusoids and peribiliary plexus in local areas.
We used the Amira software to manually segment the structure of the peribiliary plexus (Fig. 5b, c) […] Amira software (v.6.1.1, FEI) was used to distinguish hepatic vein and portal vein, and to correct errors such as missing or misidentified branches based on the cytoarchitecture information. Manual tracing of hepatic artery, bile ducts, and lymphatic vessels was also executed with the Amira […] The skeletonization of sinusoids was performed with the Amira software. The detection of the diameter of hepatic sinusoids was executed with the rayburst algorithm24 […] We used Amira to manually reconstruct the local peribiliary vascular plexus on the merged images of two channels […] The filament editor module, segmentation editor, and autoskeleton tool of Amira software were respectively used in vessel tracing, manual segmentation of peribiliary plexus, and sinusoidal skeletonization.
For Research Use Only. Not for use in diagnostic procedures.